Fast Fluorometric Method for Measuring Pendrin (SLC26A4) Cl-/I- Transport Activity
نویسندگان
چکیده
منابع مشابه
Fluorometric method for measuring serum lipase activity.
We describe a method for determining lipase (triacylglycerol acyl-hydrolase, EC 3.1.1.3) activity in serum. Emulsified olive oil in tris(hydroxymethyl)aminomethane buffer is used as substrate. The course of the enzymatic reaction is followed by measuring the decrease in fluorescence with time of a fluorescent indicator, 4-methylumbelliferone, added to the substrate, caused by the change in the ...
متن کاملThe anion exchanger pendrin (SLC26A4) and renal acid-base homeostasis.
The anion exchanger pendrin (Pds, SLC26A4) transports various anions including bicarbonate, chloride and iodide. In the kidney, pendrin is exclusively expressed on the luminal pole of bicarbonate-secretory type B intercalated cells. Genetic ablation of pendrin in mice abolishes luminal chloride-bicarbonate exchanger activity from type B intercalated cells suggesting that pendrin is the apical b...
متن کاملContinuous fluorometric method for measuring β-glucuronidase activity: comparative analysis of three fluorogenic substrates.
E. coli β-glucuronidase (GUS) activity assays are routinely used in fields such as plant molecular biology, applied microbiology and healthcare. Methods based on the optical detection of GUS using synthetic fluorogenic substrates are widely employed since they don't require expensive instrumentation and are easy to perform. In this study three fluorogenic substrates and their respective fluorop...
متن کاملDeletion of the anion exchanger Slc26a4 (pendrin) decreases apical Cl(-)/HCO3(-) exchanger activity and impairs bicarbonate secretion in kidney collecting duct.
The anion exchanger Pendrin, which is encoded by SLC26A4 (human)/Slc26a4 (mouse) gene, is localized on the apical membrane of non-acid-secreting intercalated (IC) cells in the kidney cortical collecting duct (CCD). To examine its role in the mediation of bicarbonate secretion in vivo and the apical Cl(-)/HCO(3)(-) exchanger in the kidney CCD, mice with genetic deletion of pendrin were generated...
متن کاملA Novel Method for Measuring the Quality of Temporal Integration in Public Transport Systems
Temporal coordination of services, as a crucial aspect of integration in public transport systems, has always been a big concern for transit planners and schedulers. One of the major issues in the way of coordinating transit services is the lack of a robust measure of effectiveness for assessing the quality of temporal coordination in public transport systems. Even though the network-wide summa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cellular Physiology and Biochemistry
سال: 2006
ISSN: 1015-8987,1421-9778
DOI: 10.1159/000095164